ZNova can be used to process data from a wide variety of biomolecules including proteins, oligonucleotides, peptides, etc. ProMass uses a novel deconvolution algorithm known as ZNova that produces artifact-free deconvoluted mass spectra.

Therefore, you can run a list of samples and have ProMass process them at the end or batch reprocess the list at your convenience. In fact, the normal way to run ProMass is through the Xcalibur or MassLynx sample list. ProMass was designed from the start for high-throughput applications. Overview: ProMass is an automated biomolecule deconvolution and reporting software package that is used to process ESI/LC/MS data or single ESI mass spectra acquired on Thermo Scientific’s Xcalibur or Waters MassLynx data systems.įree download page for Project SASHIMI's wolf-3.5.1-MassLynx-4.1.zip.The Sashimi project hosts the Trans-Proteomic Pipeline (TPP), a mature suite of tools for mass-spec (MS, MS/MS) based proteomics: statistical validation, quantitation, visualization, and convert. ProMass provides an automated platform for performing deconvolution of entire LC/MS data sets. These complex spectra must be “deconvoluted” or “transformed” in order to order to determine the uncharged mass of the biomolecule. This multiple charging usually results in ESI mass spectra that contain a range of charge states. On the other hand, oligonucleotides analyzed in the negative ion mode are de-protonated at multiple sites as a result of the acidic phosphate backbone. When analyzed in the positive ion mode, peptides or proteins will be protonated at multiple sites as a result of the basic amino acids and amino terminus. Screen shot of the ProMass home page Introduction: Large biomolecules when ionized by electrospray (ESI) are predominantly multiply charged.

Masslynx software download free